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propidium iodide  (Vector Laboratories)
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Vector Laboratories propidium iodide
EFV effects on amyloid pathology. (a) The content of soluble and insoluble amyloid β (Aβ) peptides in the brain homogenates of 5XFAD mice. The quantifications were carried out by ELISA. The results are presented as means ± SD of measurements in individual mice. Both female (n = 5 per treatment group, pink rectangles) and male (n = 4 per treatment group, blue rectangles) mice were used. No significant differences were found between control (Cntr) and EFV-treated (Tx) mice when female and male mice were analyzed as one group or separately. (b and d) Representative images (n = 5 male mice per treatment group) of the brain sections stained with 6E10 and Thioflavin S, respectively. Nuclei were stained with DAPI (b) or <t>propidium</t> iodide (d, false colored in blue). (c and e) Quantifications of the 6E10- and Thioflavin S-positive plagues. Area of the plaques is expressed as a percentage from the total area of either cortex or hippocampus. *P ≤ 0.05 by an unpainted, two-tailed Student’s t test. Cntr, control mice; Tx, EFV-treated mice. Scale bars, 150 μm
Propidium Iodide, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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EFV effects on amyloid pathology. (a) The content of soluble and insoluble amyloid β (Aβ) peptides in the brain homogenates of 5XFAD mice. The quantifications were carried out by ELISA. The results are presented as means ± SD of measurements in individual mice. Both female (n = 5 per treatment group, pink rectangles) and male (n = 4 per treatment group, blue rectangles) mice were used. No significant differences were found between control (Cntr) and EFV-treated (Tx) mice when female and male mice were analyzed as one group or separately. (b and d) Representative images (n = 5 male mice per treatment group) of the brain sections stained with 6E10 and Thioflavin S, respectively. Nuclei were stained with DAPI (b) or propidium iodide (d, false colored in blue). (c and e) Quantifications of the 6E10- and Thioflavin S-positive plagues. Area of the plaques is expressed as a percentage from the total area of either cortex or hippocampus. *P ≤ 0.05 by an unpainted, two-tailed Student’s t test. Cntr, control mice; Tx, EFV-treated mice. Scale bars, 150 μm

Journal: Neurotherapeutics

Article Title: CYP46A1 Activation by Efavirenz Leads to Behavioral Improvement without Significant Changes in Amyloid Plaque Load in the Brain of 5XFAD Mice

doi: 10.1007/s13311-019-00737-0

Figure Lengend Snippet: EFV effects on amyloid pathology. (a) The content of soluble and insoluble amyloid β (Aβ) peptides in the brain homogenates of 5XFAD mice. The quantifications were carried out by ELISA. The results are presented as means ± SD of measurements in individual mice. Both female (n = 5 per treatment group, pink rectangles) and male (n = 4 per treatment group, blue rectangles) mice were used. No significant differences were found between control (Cntr) and EFV-treated (Tx) mice when female and male mice were analyzed as one group or separately. (b and d) Representative images (n = 5 male mice per treatment group) of the brain sections stained with 6E10 and Thioflavin S, respectively. Nuclei were stained with DAPI (b) or propidium iodide (d, false colored in blue). (c and e) Quantifications of the 6E10- and Thioflavin S-positive plagues. Area of the plaques is expressed as a percentage from the total area of either cortex or hippocampus. *P ≤ 0.05 by an unpainted, two-tailed Student’s t test. Cntr, control mice; Tx, EFV-treated mice. Scale bars, 150 μm

Article Snippet: Slides were mounted either with ProLong Gold Antifade reagent with DAPI ( {"type":"entrez-protein","attrs":{"text":"P36935","term_id":"549826","term_text":"P36935"}} P36935 , Thermo Fisher Scientific, Waltham, MA) or with VECTASHIELD Antifade Medium with Propidium Iodide (H-1300-10; Vector Laboratories, Inc., Burlingame, CA).

Techniques: Enzyme-linked Immunosorbent Assay, Staining, Two Tailed Test